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the method comprises introducing into the prokaryotic organism an expressible nucleic acid encoding a putatively essential gene for proliferation or pathogenicity under the control of a promoter and at least one tet operator, and an expression vector comprising a nucleotide sequence encoding a modified tetracycline repressor, wherein said modified tetracycline repressor binds to a tetracycline operator sequence in a prokaryotic organism with a greater affinity in the presence of tetracycline or a tetracycline analog than in the absence of tetracycline or a tetracycline analog. (show more) ass: Standard non-polar; Column length: 2 m; Column type: Packed; Start T: 100 C; End T: 300 C; CAS no: 50486; Active phase: SE-30; Carrier gas: Nitrogen; Substrate: Chromosorb G AW DMCS (80-100 mesh); Data type: Normal alkane RI; Authors: MHA, biannually zyban uk
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